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KMID : 0191119950100030169
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Journal of Korean Medical Science
1995 Volume.10 No. 3 p.169 ~ p.175
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Identification of CD44 splice variant in Korean colorectal cancers and cell lines
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Suh SI
Baek WK/Park JW/Bae OS/Suh MH/Choe BK
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Abstract
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CD44 is a glycoprotein expressed in a wide variety of cell types. Recently expression of
some alternatively-spliced variants of CD44 transcripts (CD44v) has been suggested to play a potential role in tumor metastasis and the detection of CD44v containing exon 6 to 11 may be helpful for the diagnosis of cancers. Expressions of CD44v containing exon 6 to 11 were investigated in 20 human colorectal cancer samples, peripheral blood leukocytes isolated from colorectal cancer patients, and 4 colorectal cancer cell lines using reverse transcription-polymerase chain reaction and Southern blot analysis. The standard form of CD44 transcripts was expressed in all samples tested. CD44v containing exon 6 to 11 was expressed in 18 cases of colorectal cancers (sensitivity = 90%), 3 out of 4 cell lines, and one normal tissue (specificity = 95%). These results suggest that the expression of CD44v containing exon 6 to 11 can be regarded as tumor specific and that this marker may be helpful for the early diagnosis of colon cancers, if specimens from the early stage are available.
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